Seventy three Staphylococcus spp. isolates were recovered from
bacterial sample included: poultry , foods , sewage and soil .The antibiotic
susceptibility test to all environment isolate to Methicillin and Vancomycin
were prepared , and the result showed that 39 Staphylococcus spp. isolates
(53.42%) were resistant to Methicillin while 26 isolates (35.61%) were
resistant to Vancomycin. The range of the minimum inhibitory concentration
of Vancomycin were (2-512 μgml). Results of plasmid DNA extraction from
Methicillin and Vancomycin resistant isolates showed that some isolates have
one plasmid band. The results of the detection of Vancomycin resistance
genes (van A and van B) showed the presence of van B gene in isolate No.
ESf44 with MIC ≥ 64 μgm that isolated from raw milk , while didn’t notice
the presence of van A gene in other isolates were resistant to Vancomycin
that have been studied . This study was conducted to investigate the van B
gene within the isolated MRSA strains from food, sewage water and soil in
Baghdad.