Irritable Bowel Syndrome (IBS) is a functional disease of gastrointestinal tract (GIT) that affects 10-20% of the population worldwide and is distinguished by abdominal pain/discomfort and changes in bowel habits that is accompanied by diarrhea, constipation, or both. Control-based study was conducted on 50 Iraqi patients with IBS who were presented at private clinics for gastrointestinal diseases (GIDs), along with 20 healthy individuals matched in their age and gender to act as control group. The study lasted from November 2021 to May 2022 and aimed to determine the frequency of IBS subtypes among Iraqi patients, as well as the influence of several factors on disease’s initiation, type and severity such as age and gender, anxiety, obesity, hematological indices, and secretor status. In comparison with control group, patients showed significant higher frequency of severe anxiety and non-secretor cases particularly in those with IBS-c type. However, BMI, blood groups, and all complete blood count parameters revealed non-significant difference between patients and control groups. Among patients of different types of IBS, results showed that blood group A is highly correlated with IBS-d, and IBS-m patients, while blood type O is highly correlated with IBS-c. Also, platelet-lymphocyte ratio (PLR) is significantly associated with IBS types, which is elevated in patients with IBS-c in comparison with other types of disease. It can be concluded that people who are non-secretors and having experience of severe anxiety may be at higher risk for getting IBS. 

Irritable Bowel Syndrome (IBS) is a common gut disorder that affects approximately 11% of the global population and negatively affects the quality of life of patients and imposes a significant socioeconomic burden. Control-based study was conducted on 42 Iraqi patients with IBS who were presented at private clinics for gastrointestinal diseases (GIDs), along with 20 healthy individuals matched in their age and gender to act as control group. The study aimed to determine the frequency of IBS among Iraqi patients, as well as the influence of biomarkers on disease’s initiation, type and severity such as (IL-1ꞵ, anti- Cytolethal distending toxin B ‘Anti-CdtB’, and histamine). The results showed that constipation subtype of IBS is the most frequent among patients, and females constitute the majority of IBS patients with female: male ratio about 2.23: 1. The levels of IL-1β, anti-CdtB, and histamine are significantly elevated in the serum of IBS patients when compared to their levels in control group. In patients’ group, anti-CdtB showed significant positive correlation with their level of histamine, while correlation between anti-CdtB and IL-1β as well as between histamine and IL-1β are not significant. It can be concluded that anti-CdtB may act as a key player in IBS pathogenesis after disruption gut microbiota which needs further investigation